Abstract:

In order to make better use of Ukrainian wheat varieties, understand the quality of introduced species, the HMW-GS and gliadin subunits compositions of 16 wheat cultivars from Ukraine were analyzed by SDS-PAGE and A-PAGE. The results showed that a total of seven subunit combinations were examined on Glu-1 loci, and (1, 7+8, 5+10) were the major combination type with frequencies of 37.5%, followed by combinations (1, 2+12, 6+8) and (1, 7+9, 5+10), the frequencies were 18.75%. Three kinds of HMW-GS on Glu-A1 were examined, the frequency of 1 was the highest (75%). There were three kinds of HMW-GS on Glu-B1, and subunits 7+8 were the major types with frequencies of 43.75%. Three types were detected on Glu-D1, and 5+10 appears more frequently (68.75%). Aspects of gliadin, 33 gliadin band patterns were encoded by Gli-1 locus and there were 41 protein bands with different mobility. 6, 5, 5 alleles were at Gli-A1, Gli-B1, Gli-D1, and there were 6 ,5, 6 alleles at Gli-A2, Gli-B2, Gli-D2. Good-quality gliadin alleles Gli-B1b was the most frequencies alleles (43.75%). Maybe these varieties can be used as a valuable material for breeding.