Abstract:

The key chalcone synthase (CHS) gene was cloned in tartary buckwheat, and its evolution status was evaluated, which would play an important role for molecular assistant breeding for the excellent Fagopyrum tataricum accessions with high flavonoids content. The gene was cloned by rapid amplification of cDNA Ends (RACE) and analyzed the basic physicochemical properties and homology by bioinformatics methods. Moreover, the phylogenetic tree of chalcone synthase family was constructed by neighbor-joining. Full length of CHS cDNA was 1250 bp containing 241 bp 3'UTR and 185 bp 5'UTR. Isoelectric point (pI) and molecular weight (Mr) were 5.33 and 35340.75 Da, respectively. Meanwhile, 975 bp open reading frame (ORF) was cloned. We predicted the gene encoding 325 amino acid residues. Amino acid sequences alignment results showed that chalcone synthase in tartary buckwheat had higher similarity with Polygonum cuspidatum. The phylogenetic tree showed that chalcone synthase of tartary buckwheat had one same origin with other dicots. The genetic relationships were closed among Gypsophila paniculata, Polygonum cuspidatum, F. dibotrys, F. esculentum, F. tataricum. We obtained the cDNA of CHS in tartary buckwheat, cloned the whole ORF, determine the status and direction of the enzyme in tartary buckwheat.