Abstract:

In order to provide a trial exploration for studying the fluorescent features of different biological materials and opening up new areas of fluorescent histology, Drosophila melanogaste ovaries were used as experimental materials. The ovarioles were firstly dissected, and then treated in three ways: directly squashed material, squashed material after fixation with paraformaldehyde, and squashed material stained with five different deys after paraformaldehyde fixation. The squashes were observed and photographed under fluorescent microscope in bright field, and fluorescent fields after excitation with green, blue and UV light, respectively. The results showed that Drosophila ovarioles possesses weak autofluorescence, paraformaldehyde fixation endowed the material evident fluorescent features, and different staining endowed the material various fluorescent characteristics. Based on the above results, the following conclusion can be reached: fixatives and dyes can endow Drosophila ovarioles and different cell types, or even different cellular components specific fluorescent characteristics. Fluorescent techniques might open a bright applying field for the traditional histology.