Abstract:

The main influential factors of SRAP reaction system were optimized for genomic DNA in wax apple (Syzygium samarangens) by orthogonal design. The results showed that the optimum concentration was 1×Buffer, Mg2+ 3.0 mmol/L, dNTPs 0.3 mmol/L, primer 0.4 μmol/L, Taq DNA polymerase 0.5 U, 40 ng template DNA with a total volume of 20 μL reaction solution. The molecular identification of 7 varieties of wax apple were achieved by 64 SRAP primer combinations with this optimum system which could provide clear, reliable and repeatable abundant polymorphisms molecular markers.