Abstract:

The aim was to study the bacteriostatic action and influencing factors of extract made from Pinellia ternate, to get its antimicrobial spectrum. The reflux extraction method was used to extract the active material in Pinellia ternata by using 95% ethanol, and agar diffusion method and liquid mixed culture method were used to test the MIC (minimal inhibitory concentration) of Pinellia ternata against the bacterium. The rate of Pinellia ternata extract was 5.24% with 75% ethanol while which was 2.73% with 95% ethanol. Minimal inhibition concentrations (MIC) against several microorganisms including bacterium and fungi were analyzed. The minimum inhibitory concentrations of tested strains were as follows: which of E. coli was 25 mg/mL, of Pseudomonas putida 12.5 mg/mL, was of Staphylococcus aureus was 12.5 mg/mL, of Micrococcus luteus was 12.5 mg/mL, of Bacillus subtilis was 10 mg/mL, of Saccharomyces pombe was 20 mg/mL, of Saccharomyces cerevisiae was 10 mg/mL, of Aspergillus niger was 12.5mg/mL, of Melon fusarium wilt was 25 mg/mL. The results showed that the juice of Pinellia ternata exerted different effect on a series of bacterium and fungi. The juice of Pinellia ternate extracted by alcohol could effectively inhibit the growth of Gram-positive and Gram-negative bacterium, but the bacteriostatic action of Pinellia ternata against fungi were not clear enough. It could conclude that the bacteriostatic effect of Pinellia ternate juice was enhanced with the increase of extract concentration.