Abstract:

In order to obtain the high yield and quality of Cordyceps militaris, it was the main influencing factors that affected growth rate and strong of mycelia. The author found out the optimal growth index of mycelia by single factor experiment, and optimization various additions of medium components by the uniform test. Research showed that lactose and peptone were the best carbon and nitrogen source for the cultivation of mycelia. Optimization of medium components of mycelium obtained: peptone was 2.5 g/L, lactose was 39.9 g/L, KH2PO4 was 0.5 g/L, MgSO4?7H2O was 3.2 g/L and CaCl2 was 0.3 g/L; the suitable temperature for the mycelium of this fungi growth was ranged from 15-28℃ with the optimum temperature being 25℃, the suitable pH was ranged from 5-9 with the optimum pH being 7.5, inorganic salts had little effect on the mycelium growth rate, but it was to a certain extent mycelial growth potential, light could inhibit the growth of mycelium, the dark culture was appropriate. In these conditions through the experiments demonstrated that the growth speed of mycelia could reach 4.0 mm/d, and Cordyceps colony presented a thick, white, hairchested and luster.