Abstract:

The aim was to clarify the effects of plant allelochemicals on the detoxification enzyme activities in aphid. The effects of 2-tridecanone and quercetin on the specific activity of glutathione S-transferases (GSTs) and carboxylesterase (CarE) in aphid were investigated by using feeding and fumigation treatments. The results showed that the aphids fed on artificial diet added 0.1% 2-tridecanone and 0.01% quercetin showed the maximum activities of GSTs and CarE, respectively. The maximum activity of GSTs was at 24 h and the difference was significant compared to the control. The maximum activities of CarE measured using α-NA and β-NA as substrates were at 48 h, and the difference were significant compared the control. The activities were increased by 2.45-fold and 2.53-fold respectively compared to the control. Thus, the activities of GSTs and CarE were induced on dosage significantly, by feeding 2-tridecanone and quercetin respectively, and on time significantly by 2-tridecanone fumigation.