Abstract:

The effect of explants collection time, different sterilization methods, antibiotics to inner bacteria, different medium and growth regulators on adventitious buds regeneration of Ziziphus jujube ‘Junzao’ using nodal explants from mature trees was studied and evaluated to establish a high adventitious buds regeneration system for the use of genetic transformation technology for genetic improvement. The explants were collected in May was best compare to other period. Intermittent sterilization method and adding 480 mg/L penicillin were optimum to control the contamination. Highest regeneration rate was obtained on 1/2MS with TDZ 0.5 mg/L, IBA 0.2 mg/L with the nodal explants inoculation straightly.