Abstract:

The purpose of the present research was to analysis the genetic diversity of Canarium album L. germplasm resources in eastern Guangdong, which provided reference for protect and research in Canarium album L. germplasm resources. In the present study, genetic diversity of 64 accessions Canarium album were investigated by using ISSR markers. A total of 128 bands were generated, of which 99 bands were polymorphic, the percentage of polymorphic loci was 77.34%. Population genetics analysis showed that the average observed number of alleles (Na), effective number of alleles (Ne), Nei’s gene diversity (H)and Shannon’s information index (I) were, 1.7734±0.4203, 1.4823±0.3888, 0.2746±0.1975, and 0.4072±0.2721 respectively. The genetic similarity coefficient among 64 accessions ranged from 0.5714 to 0.9379, and the average was 0.7793. 64 accessions were clustered into 5 groups by the method of clustering analysis, and 5 groups by the method of principal coordinate analysis. The result indicated that the genetic diversity of Canarium album germplasm was low in these areas, and the results of molecular clusters had no correlation with the geographical origin of accessions.