Abstract:

In the process of separating the soft rot pathogen in konjac, Wh2 was got which strongly inhibited the soft rot pathogen. According to the morphology characteristics, physiology and biochemistry tests, the comparison of 16S rDNA sequence, and HPLC-MS analysis, the Wh2 was similar Bacillus amyloliquefaciens. MS showed the active fraction was at the retention time of 18.92 min and the corresponding ultraviolet absorption peak wavelength was 229.5 nm. The molecular mass was 425 Da.