Abstract:

In order to identify cotton variety and test seed purity at the molecular level, gene purity and variety specificity of 51 cotton (G.hirsumtum L.) varieties were characterized by SSR primer pairs with high polymorphisms and stable amplified bands. The results showed that: most (69%) of the tested varieties with gene purity greater than 90%, while 12% of them with gene purity less than 80%. Genetic cluster analysis based on the marker genotypes indicated that all of the tested varieties were divided into two groups and four sub-groups. And 83% of the tested varieties were distinct one another using a genetic distance threshold of 0.01. All the results suggested that the SSR markers were suitable for and effective on characterization of gene purity and variety specificity of cotton varieties.