Abstract:

In order to study the mechanism of wheat cold resistance, and discover genes associated with mutant cold resistance, a cold resistance mutant, obtained by spaceflight mutagenesis on the seeds of wheat variety ‘Han6172’, and the DNA of cold resistance mutant and contrast ‘Han6172’ were compared by SRAP technique. 380 pairs of primers were screened, 6 pairs of them had polymorphisms between mutant and contrast, the rate was 1.58%, and this data indicated that there were no obvious DNA differences between mutant and contrast. Six specific fragments were obtained, 3 fragments of them were amplified in mutant. Homology analysis in GenBank showed that Me3-Em7-Mt, Me4-Em11-CK, Me7-Em19-CK and Me6-Em9-Mt all had homologous sequences with wheat chromosome 3B-specific BAC library, and this result indicated that the gene and regulator sequences associated with mutant cold resistance might locate on 3B chromosome.