Abstract: An in vitro propagation protocol based on bulb proliferation has been developed for Lilium davidii var. unicolor . BA 0.9 mg/L + NAA 0.1~0.2 mg/L was suitable for bulb proliferation .It seemed that high or low total ion and nitrogen concentration affected bulb proliferation. N6 was optimal basal medium for bulb proliferation. Improving sugars concentration enhanced the multiplication efficiency. The multiplication efficiency of 40 g/L Sucrose was higher than others. The best rooting response was obtained on 1/2 MS medium supplemented with 0.1 mg/L IBA, on this medium; the rooting frequency was 100%. The survival percentage was 90% when rooting plantlets were transplanted in perlite and soil.