Abstract:

The aim was to study the biological control of asparagus stem blight. In this paper, one antagonistic strain was isolated from the greenhouse soil of asparagus planted which was identified as Bacillus cereus by morphological properties, culture characteristics, physic-chemical indexes and molecular analyses. Strain KJ-1 could strong inhibit the mycelia growth of asparagus stem blight and the inhibition rate reached 65% when the fermentation was diluted (1:4). Fermentation of KJ-1 was applied into greenhouse soil of asparagus, which increased the number of soil microbe that was 1.38 times than CK, the numbers of bacteria and fungi all were 1.52 times than CK, ratio of B/F was increased slightly. The microbe diversity of metabolic group was also changed, AWCD and species richness (S) were all higher than those of CK, and the diversity of AWCD between test and CK was significant (P<0.01). It indicated that fermentation of KJ-1 enhanced the number and biodiversity of microorganism in greenhouse soil of asparagus planted.