Abstract:

For better enrichment and purity of napropamide in tobacco leaves, the molecular imprinted polymers as a solid phase extraction column coupling to HPLC was studied. The molecular imprinted polymers (MIPs) was synthesized by polymerization for 24 hours using napropamide as template, methacrylic acid (MAA) as monomer, ethyleneglycol dimethacrylate (EDMA) as crosslinker and azodiisobutyronitrile (AIBN) as initiator at 70℃ in column. This monolithic column could specially recognize napropamide by affinity. The column was used as a solid phase extraction (SPE) pillar for affinity extraction and enrichment of napropamide residue in acetonitrile extract of tobacco leaves in HPLC system. Then, the sample was analyzed by reversed phase-HPLC. The SPE-HPLC method showed good recovery [(92.3±2.1)%], good reproducibility (RSD 2.76%), excellent linear correlation (R2>0.9996) and low detection limit (1.0 ng/g). In conclusion, the napropamide molecular imprinted polymers monolithic column was a good solid phase extraction affinity column for enrichment and purity , and the MIPs-SPE-HPLC method established was suitable for the analysis of napropamide residue in tobacco leaves.