Abstract:

The aim was to study broad-spectrum anti-aphid gene in sorghum and sorghum aphid interactions in the molecular genetic basis and mechanism, and to explore and use the information of aphid resistance genes. In this study, ‘Henong16’ with aphid-resistance genes and ‘Qian3’ susceptible to sorghum aphid were used as the tester and the driver and vice versa to construct SSH library, so that the aphid resistance genes were enriched effectively, then the differential gene sequence was analyzed. 200 positive clones were randomly selected and sequenced. 18 open reading frame (ORF) sequences were found through the NCBI ORF Finder online prediction. Then the 18 ORF sequences were blasted and analyzed using online Blastx. The results showed that the 18 ORF sequences were highly related with putative oxygen-evolving enhancer protein in chloroplast, adenylate cyclase-associated protein, ribosomal protein, putative senescence-associated protein. The contigs without ORF were blasted and analyzed using online Blastx and Blastn, and 66 ESTs had highly homologous to genes sequences with known disease resistance in many plants, such as ribosomal protein, transport membrane protein, NBS-LRR disease resistance protein family-1, zinc finger protein, ATP synthase, NADH dehydrogenase, retrotransposon, Adenylyl cyclase-associated protein, and bZIP transcription factor etc.. These homologous genes involved in secondary metabolism, energy metabolism, membrane transport, signal transduction and transcription regulation and so on. The SSH library of sorghum aphid resistance gene was constructed successfully, which indicated that the SSH technique in the mechanism of aphid resistance of sorghum was a feasibility study. The results laid the foundation for further studying on sorghum aphid-resistance gene function.