Abstract:

To study the specific antimicrobial effect in vitro caused by antibody of E.hemannii. The polyclonal rabbit antibody against E.hermannii was prepared and puried with Protein Gresin subsequently. Scanning electron microscope and nutrient agar media were used to evaluate the mixture of puried antibody and E.hermannii at different time points. Twenty Balb/c rats infected by E.hermannii through wound on their backs were used to evaluate the therapy effect of the prepared antibody of E.hermannii. The result showed that E.hermannii began to lyse at 20 minutes and was disintegrated completely at 80 minutes. In addition, there was no alive E.hermannii growed on nutrient agars where the mixture was plated. Infection assay against rats showed that the recovery time of infected rats which were treated by prepared antibody was shorter than those of without antibody introduced. Prepared polyclonal antibody against E.hermannii could be used as one of immune therapy methods for clinical cases caused by E.hermannii.