Abstract:

In order to identify germplasm resources of Fagopyrum tataricum, in this study the ITS general primers of sequence was used, F. tataricum genomic DNA as a template was amplified by PCR out of ITS sequences (internal translation spacer), the cloned sequence was based on 5 cultivars of F. Tataricum for molecular identification and molecular analysis of genetic relationship. The results showed that: (1) five materials were a small variation of ITS sequences, the length of sequences between 584-590 bp, G+C content was high up to 67.5%-68.5%. (2) Using different clustering analysis showed that ‘Jiande’ F. Tataricum and ‘Honghua’ F. Tataricum, ‘Xi 2’ F. Tataricum and ‘Xi 3’ F. Tataricumt had a closed genetic relationship.