Abstract:

The objective of this study is to find a simple method to induce spawning. Exogenous hormones in this experiment include HCG and LHRH-A2 and DOM. Every loach is in accordance with basic amount of HCG250 IU and LHRH-A22 μg and DOM1 mg. Salt is used as aided agent for soaking and salad oil is used to eliminate foam. We studied the effect of different soaking time and different concentrations and different salinity on propagation of loach. The total amount of loach for soaking experiment is 13.987 kg and 900 loaches were injected into exogenous hormones and 802.18 thousand spawning eggs were collected. The rate of inducing spawning was from 0% to 100%. The average spawning rate was 34.66% and 714.2 thousand loach juveniles were got and the hatching rate was from 45.6% to 93.3%. The average value of hatching rate was 81.21%. The rate of induce spawning induced by exogenous hormones is 80% and there is no significant difference on hatching rate between injection hormones and hormones soaking method. This study indicates that hormones soaking method is likely a new convenient, time-saving and promising method for loach.