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Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (6): 58-62.

Special Issue: 资源与环境 马铃薯

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Genetic Polymorphism of Cassava Germplasm SSR Molecular Markers

  

  • Received:2011-08-31 Revised:2011-11-13 Online:2012-02-25 Published:2012-02-25

Abstract:

In order to analyze the genetic diversity in 80 cassava accessions, among which 25 were Guangxi Subtropical Crops Research Institute introduced from Thailand, Philippine, Burma and Indonesian, and 65 varieties belong to Hainan and this unit etc, by using 12 pairs of SSR markers, 119 loci were obtained totally, the fragment size of PCR products ranging from 100 bp to 300 bp, 4 to 16 alleles were detected by each pair of primer, with an average of 10. According to the genetic similarity coefficient among different strains and the UPGMA method, with the similarity coefficient criteria of 0.46 these 80 accessions were clustered into 2 groups. These results indicated that the introduction of foreign resources and the breeding varieties of Guangxi Subtropical Crops Research Institute enriched cassava germplasm bank in China, and expanded our country’s cassava breeding basis. It also provided the genetic basis for the breeding of new varieties of cassava or the collection and management of germplasm resources.