Abstract:

For adapting the development of free pollution agriculture and aiming at biological control of Blumeria graminis f. sp. Tritici, we isolated an antagonistic strain A2 that was identified as Bacillus subtilis from soil of protected farmland. The strain was identified based on morphological，physical characteristics and the homological analysis of 16S rDNA sequence. Meanwhile, testing the control efficacy of Blumeria graminis f. sp. Tritici with the treatment of spraying clear water as control. The results showed that: A2 strain was road-shaped bacteria and gram-positive, could produce bacterial endospores of oval, amylase, fluidity glutin, and showed the positive reaction in the litmus milk test. In pot plant experiments, A2 strain and its culture supernatant showed significant control efficacies against Blumeria graminis f. sp. Tritici, and the control efficacy of admixture of them was much better. The control efficacy of fermentation broth of A2 strain achieved 72.22%, when the control efficacy of culture supernatant of A2 strain was 29.24%. The conclusion was drawn that: it was feasible for A2 to be applied in biological control and could provide scientific basis for the production of new free pollution biopesticide.