Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (15): 36-41.doi: 10.11924/j.issn.1000-6850.2011-3123

Special Issue: 棉花

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AFLP Fingerprinting Analysis of 31 Cultivars Attending the National Regional Trial

  

  • Received:2011-10-26 Revised:2011-12-20 Online:2012-05-25 Published:2012-05-25

Abstract:

For exploring the advantage of AFLP molecular marker in constructing the cotton fingerprinting, 31 cultivars of conventional cottons in team B, hybrid cottons in team C attending the 2009 and 2010 national regional trials were used for DNA fingerprinting. Two pairs of core AFLP primer were used for polymorphism analysis. Forty-four polymorphic bands were obtained which took 28.39% of total amplified bands. A primary DNA fingerprint database was established with 44 polymorphic bands. The cluster analysis showed that, all tested-cultivars could be completely identified at 0.92 similarity index level, and conventional cottons almost cluster together preferentially in different extent, so as hybrid cottons.