Abstract:

In order to solve the problem of soybean herbicide chlorimuron-ethy residues in soil for long time. To provide actinomyces source for bioremediation of soil contaminated by chlorimuron-ethy. A bacteria which was able to degrade chlorimuron-ethyl and could grow using chlorimuron-ethyl as the sole nitrogen, was isolated from the soil of applying with chlorimuron-ethyl for many years by enrichment culture, which was named SN10. Based on physiological and biochemical characteristics and 16S rRNA sequence, the strain was identified preliminarily as Bacillus cereus. The influencing factors (temperature, pH, inoculation amount and incubation time) were studied with bacteria growth mass and degradation ration as indexes. The results showed that the optimal degradation ratio occurred at the condition of 28℃, pH 7, inoculation amount of 7%, incubation time 4 days. Under these conditions, 81.2% chlorimuron-ethyl had been degraded. By chlorimuron-ethyl degradation bacteria evaluation of the repair test, bacteria and drug treatment of maize seed germination and plant height were higher than only drug treatment, SN10 degraded obviously chlorimuron-ethyl in soil.