Abstract:

For the rapid detection of Bacillus bacterial concentration, the spectrophotometric method was used to detect Bacillus cereus concentration, instead of the traditional time-consuming and laborious biological cell assay. In this study, we took Bacillus cereus cultured in different pH as an example, measured OD value of Bacillus cereus cultured in different pH by visible spectrophotometry, water and culture medium as the blank control respectively, and its corresponding cell concentration of Bacillus cereus was compared. By bio-statistical software DPS, the correlation between concentration (Y) and its absorbance (X) (water control) of Bacillus cereus was very good. The absorbance method could be used as a rapid detection of Bacillus spp. cell concentration indicators. In summary, the experimental results demonstrated that Spectrophotometric detection can replace the concentration of Bacillus cereus expressing the biological characteristics of Bacillus, and this method was simple, fast and reliable.