Abstract:

Culture method of microbes could obviously affect their metabolites and bioactivities. The bactericidal activity of three kinds of organic crude extracts from Lepista sordida with different culture methods against Xanthomonas oryzae was explored in this paper. These organic crude extracts were prepared from Lepista sordida cultured by solid plate, liquid surface fermentation and liquid submerged fermentation with the same medium of PD. It was showed that, ethyl acetate extract from the liquid of L.sordida by liquid submerged fermentation had significantly bactericidal activity against Xanthomonas oryzae, the pathogen of rice bacterial leaf streak, with the minimum inhibitory amount of 6.25 μg. However, the organic solvent extracts from the mycelia of L. sordida cultured by liquid submerged fermentation, from the mycelia together with medium of L. sordida by solid plate and from the liquid of L. sordida by surface fermentatarion had no bactericidal activity against Xanthomonas oryzae. In a conclusion, the bioactivity component could be obtained from the liquid of L.sordida by liquid submerged fermentation.