Abstract:

In order to conserve and multiply germplasm rescources of Mei flower, the gern-free system of embryo-culture was constructed in seeds of four cultivars. The contents of this paper were as follows: we conducted immature embryos culture of Prunus mume ‘Danhanhong’, ‘Nanjing Hongxu’, ‘Shuangbi Chuizhi’, ‘Fenban’ derived from pollination. In addition, using leaf of seeding cultivation as explants, the efficient of callus culture was conducted. Results showed that the perfect culture medium was MS+6-BA 1.0 mg/L+IBA 0.2 mg/L. The optimum callus culture was MS+2,4-D 1.0 mg/L and perfect leafage was young period. Germination Rate of cultivars is different. The difficulty was single-lobe Mei Branch < hanger wood Mei Branch < cinnabar Mei Branch.