Abstract:

In order to obtain the recombinant protein NPY containning TAT protein transduction domain and study the bioactive of the recombinant protein. NPY gene was cloned from the Calf Hypothalamus tissue by the molecular biology techniques. Coincidence rate was 100% by genetic test. TAT-protein gene was inserted into bovine NPY gene sequences and expression vector pGEX-3X-NPY-TAT was constructed, then the recombinant plasmids of pGEX-3X-NPY-PTD was induced in E. coli DE3 by addition of IPTG. The expressed fusion protein was identified, It was optimized and the bioactive test was carried out by CAM. The results showed that the bovine NPY gene of TAT protein transduction domain was expressed, and the expressed fusion protein had biological activity. Conclusion the study provided important base for the animal experiment.