Abstract:

The latest research showed the obstacle of mycorrhizae formation was lack of some signal material that can induce mycorrhizal fungi mycelial to grow and branch. The co-cultures of callus of Ostryopsis davidiana and its mycorrhizal fungi were established for improving mycorrhizal fungi cultivation methods. And the secondary metabolites of the co-culture were studied. Using the dry weight method, the influence of the O. davidiana callus and homogenate and secretion of callus on the mycelium growth of Cenococcum geophilum Fr. (Cg) was investigated in vitro. The results showed that, the two strains which co-cultured with the host callus could obviously promote the fungal hyphae germination and growth. At the same time, homogenate and secretion of callus could also facilitate early mycelia growth. While the most effective method was the co-cultures, and the single colony dry maximum biomass of each Cg strain reached to 63.5 mg and 45.2 mg. The research of preliminary got a good cultural method of ectomycorrhizal fungi mycelium which could evidently upgrade the growth of the hyphae. The O. davidiana callus cells surely secreted some biochemical substances that promoted the growth and branch of hyphae in the progress of the growth.