Abstract: To produce a rapid Newcastle disease virus (NDV) pathogen detection method. BALB/c mice were immunized with HN recombination protein and the mouse splenic cells were fused with SP2/0 cells, hybridoma cell stably secreting anti-HA McAb was screened by ELISA designated 4F8, the immunoglobulin type of McAb identification shown was IgG2a type with κ chain. An antigen capture ELISA (AC-ELISA) was developed for detection of NDV using polyclonal antibody as capture antibody and specific monoclonal antibody 4F8 as detecting antibody. The AC-ELISA showed no cross-reaction with other four avian viruses (EDSV, ITLV, IBV, IBDV), The sensitivity four times higher than HA test, Comparing with RT-PCR, the concordance, sensitivity, specificity was 95.6%, 93.3%, 96.1%, respectively. The NDV AC-ELISA had good specificity, sensitivity and repeatability, could be used for diagnosing NDV infections.