Abstract:

Fermentation medium optimization of Trehalose synthase from pET-30a(+)/TreS by the response surface method. Firstly we evaluated the effect of eight ingredients on Trehalose synthase by Plackett-Burman design evaluation method. Then we determined value range change in the central composite design of important ingredients by the most steep hills experiment. Lastly we found out optimal concentration of important components by central composite design of response surface method. The results showed that maltose, protein peptone, beef cream were important ingredients, which had significant influence on the enzyme activity (P<0.05). Their optimal concentration were the maltose 22.07 g/L, protein peptone 5.46 g/L, beef cream 2.16 g/L. After the optimization of culture medium, enzyme activity could reach 0.826 U/mL, which were raised two times than enzyme activity of foundation fermentation cultivation of recombinant strains. Mathematical statistics and analysis of the experimental design method was applied successfully to optimization of fermentation of Trehalose synthase from pET-30a(+)/TreS, which laid a theoretical foundation for the industrial fermentation Trehalose synthase.