Abstract:

Glucosinolate-myrosinase is a kind of enzymes that degrade glycosidase, the express of this gene is tissue-specific. Research showed that, the new myrosinase TGG4 and TGG5 are found in Arabidopsis thaliana root. In order to find the function of TGG4 and TGG5, we extracted the myrosinase from ecological Col-0 root and the yeast contained recombinant protein, determined the enzyme activity condition and analyzed the different. It suggested that, the root myrosinase need higher ascorbic acid active concentration, it was about 2 mmol/L, the best reaction temperature and pH range were the same, for root myrosinase, the temperature was 37℃, and the pH was 4.5, for all myrosinase the NaCl had a strong inhibition of effect. The author believed that, root myrosinase did not work in salinity and themostability, and the enzyme also could not help root against disease and insect attack, but it might be play an important role in root growth.