Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (15): 163-169.doi: 10.11924/j.issn.1000-6850.2012-0116
Special Issue: 生物技术
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The aim was to explore the role of catalases of Fusarium oxysporum f. sp. Cubense (race 4, FOC4) in pathogenesis. Titanium sulfate and hydroxylamine oxidation methods were used to detect the content changes of H2O2 and O2?- in the roots infected by FOC4. At the same time, two catalases in FOC4 were cloned and characterized by using genome information of Fusarium oxysporum (strain Fo5176) from Genbank. And then, Gene expression patterns induced by exogenous H2O2 and Musa paradisiaca were detected by RT-PCR. The result showed that the infected by FOC4 could cause oxidative burst in the roots of banana seedlings. Phylogenetic analysis showed that two catalases belonged to CatalaseA and catalaseC. The expression of 2 catalases had increased after induced by exogenous H2O2 and Musa paradisiaca. Foc4 CatalaseA might be one of the catalases which played a major role in eliminating the strong oxidative stress environment.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2012-0116
https://www.casb.org.cn/EN/Y2012/V28/I15/163