Abstract:

To find a DNA extract method suitable to Opisthopappus taihangensis and obtain a few ISSR markers for O. taihangensis, the total DNA was isolated from the O. taihangensis by the common CTAB method, improved CTAB method and SDS method, then O. taihangensis DNA as template, ISSR primers were screened and the PCR conditions were optimized. The results showed the improved CTAB method got a high production rate of the extracts, and that was pure with little degradation. These productions were good templates for ISSR molecular markers. Then, out of 50 ISSR primers, 15 ISSR primers were screened which could generate clear, stable bands. By setting gradient temperatures for each primer, reaction conditions were optimized. Eventually, population samples were detected by primer UBC848, clear and stable bands were gotten. These results showed the selected ISSR primers were suitable for the study on population genetics of O. taihangensis.