Abstract:

In order to study the role of the key enzyme gene AGPase in starch synthesis, according to the other species’ AGPase small subunit gene of the GenBank, we got 909 bp gene fragment with 3’ end from FX01. Through sequence similarity search results showed that, its nucleotide sequence with GenBank landing Ricinus communis ,Populus trichocarpa, Citrus sinensis, Ipomoea batatas, Citrus unshiu and Glycine max sequence homologily, respectively of 91%, 91%, 87%, 86%, 86% and 85%. AGPase gene antisense expression vector PBI121-AGPss was constructed based on the known vector pBI121, and transferred it into Agrobaterium tumefaciens to get the transformed bacteria. It made the foundation for further study of cassava starch synthesis-related gene regulation, GM and gene function.