Abstract:

This study aims to analyze the genetic structure and screen the markers associated with growth by the microsatellite technology. The genetic structure of one breeding population of Japanese scallop (Patinopecten yessoensis) was analyzed using 19 polymorphic microsatellite markers. The results showed that a total of 60 alleles were detected for 19 loci, and the number of alleles ranged from 2 to 4. The average effective number of alleles was 3.26; observed heterozygosity and expected heterozygosity were 0.2952 and 0.6352, respectively. The mean polymorphism information content was 0.5671. The probability value of Chi-square test showed that more than half of the 19 loci have significantly deviated from Hardy-Weinberg equilibrium (P<0.01). The SPSS 16.0 was used to analyze the effect of these 19 microsatellites on body weight, shell length, width and height. Results indicated that DQ679223 had a significant impact on shell width; EF056526 had a significant impact on body weight, shell length, width and height; DQ221720 had a significant impact on body weight shell width and height, FJ262409 had a significant impact on body weight. The results show that the genetic diversity of this population was high, what’s more, the markers related with the growth can be used to assist the breeding and improve the efficiency.