Abstract:

In this study, in order to directly use MabinlinⅡ in functional food, the MabinlinⅡ gene was reconstructed and then the recombinant MabinlinⅡ gene was inserted into the food-grade expression vector. After electroporation and lactose utilization screening, the food-grade inducible expression system for recombinant plant sweet protein MabinlinⅡ in L. lactis was constructed. The expression of the induced recombinant MabinlinⅡ was in a low level. The western blotting results demonstrated that the recombinant MabinlinⅡ protein was successfully expressed by the recombinant food-grade L. lactis when the inducible expression system was induced by Nisin. This research showed the feasibility of constructing a food-grade L. lactis dairy product strategy by removing the need for addition of sugar and contributing to the value of functional food.