Abstract:

F1 could be identified by DNA marker technology in genome. To provide the basis for F2 group construction and gene location of mutants, F1 hybridized with rice mutants were identified by SSR markers. Mutants of albefaction from R30 and of narrow leaf from ‘Luhui 17’ induced by EMS were used as parents respectively in two cross-combinations. The F1 plants from the two combinations were respectively identified by three SSR markers. The four F1 plants (code 1-4) from mutation of albefaction from R30בLuhui 17’ were identified by RM6105. The code 4 showed complementary marker type of both parents. This indicated it was real F1. The marker types of other three were the same as its female parent. They were self-pollinated for female parent. The results of identification by RM6965 and RM5349 were consistent with that by RM6105. Three (code 9, 12 and 15) from the fourteen F1 (code 5-18) from R30×Mutation of narrow leaf from ‘Luhui 17’ were identified to be real F1. The F1 crossed with R30 and ‘Luhui 17’ could be identified respectively by the three markers of RM6965, RM5349 and RM6105 and the results were same. The F1 identification with molecular marker could be applied in cross-breeding.