Abstract:

In order to identify miRNA (microRNA) in Cassava, to understand its mechanism of accumulation in cassava. In this study, lots of miRNAs were identificated in Cassava by Solexa sequencing。By using a new PCR based assay platform (Multiplexed RT assay), miRNAs were verified in cassava. 93 conserved miRNAs comprising 19 families and 74 novel miRNAs were verified through sequencing and bioinformatics analysing three small RNA library, 83 miRNAs were verified by Multiplexed RT assay. 6 of miRNA, miR172d, miR396c, miR398a, miR398b, miR399e and miR399f, were specifically expressed in Arg7 tuber; 13 in leaves; and 3 in fibrous roots. These miRNAs provides a new perspective to intensive study tuber development and the mechanism of starch accumulation in the cassava.