Abstract:

Using differentiated test tube seedlings of Crataegus cultivar as experimental material, we investigated the effect of different light spectrum on differentiation, rooting, and growth after transplantation. We hope this research could shorten production cycle of plant tissue culture, enhance the quality of tissue seedlings, provide shortcut to fast propagation of other plant species, and offer theoretical basis and data support for the effects of light spectrum on plant growth. The results showed that the 7︰9 ratio of red and blue light quality had the greatest impact on Crataegus differentiation and rooting in vitro, for the differentiation was earlier and the rate reached 89.7%, the rooting had more number and strong, the rate reached 44.7%. After 4 days transplanting to the matrix which mixed with the turf︰vermiculite︰perlite in 2︰1︰1. Observed after 25 days, the red and blue 7︰9 seedlings grew best, tidiness and high strong; root length reached the maximum difference under the red light, that indicated the long waves promoted the plant extension length growth; their root fresh mass and root length decreased under blue light, which showed short wavelength light had less impacts on Crataegus growth; comprehensive the differentiation, rooting and transplanting, we obtained the results for the 7︰9 ratio of red and blue was the most appropriate light quality on growth of Crataegus.