Abstract:

For research and development of biological agents, polyphenol oxidase (PPO) was extracted from Sucra jujube chu and the properties of PPO were studied. The results indicated that the 5.8-fold purification was achieved when the crude enzyme was treated with 40% saturated (NH4)2SO4. The substrate, 10 mmol/L of catechol couldn’t limit enzymatic reaction speed in the enzyme reaction. The results also reported the optimum pH was 7.0 and the best temperature was 37℃ for the tested PPO. At the same time, PPO from Sucra jujube chu couldn’t withstand the high temperatures and PPO activity would reduce along with time extension. PPO would be inactive completely after incubation in the temperature of 70℃ for 9 min.