Abstract: In order to analyze the fertility expression of one kind of super hybrid rice, we selected the photo-/ thermo-sensitive genetic male lines‘Pei’ai 64S’, three different climatic regions were Hainan, Hunan and Jiangxi. The results showed that, the same CMS in different climatic regions there was a big difference in their sensitive period,‘Pei’ai 64S’in Changsha the sensitive periods in the 0-8 days before flowering, ieⅦ,Ⅷperiod. In Haikou theⅢ,Ⅳperiods were the sensitive period; In Jiangxi, theⅢ,Ⅳperiods were the sensitive period. Under natural conditions, CMS 64S in Changsha in 2010 from July 17 to October 7, pollen sterility almost all of > 98.0%, other time fluctuate large. In Haikou, from May 11 to October 18 in 2010. The pollen sterility were greater than 99.50% from 11 May to 18 October, seed-setting rates all less than 0.50% . In Nanchang in 2010 from July 12 to October 11, pollen sterility almost all of > 97.0% , seed-setting rates fluctuate between from 0.00% to 0.85%. Thus, the seed in Haikou, the most secure, and stable sterility longest, as long as five months.