Genetic Transformation of Cassava with Its SBEⅠ Antisense Gene

doi:10.11924/j.issn.1000-6850.2012-2104

Abstract

Abstract: This study aimed to gain transgenic‘SC-8’cassava with the transformation of starch branching enzyme (SBEⅠ) antisense gene, and provide basis for using transgenic technology in the improvement of cassava starch quality. Based on the cassava regeneration system which could take the embryoid cotyledon to complete plants, the SBEⅠantisense gene was transformed by Agrobacterium-mediated transformation and the tuber-specific promoter Sporamin was used. 517‘SC-8’cassava cotyledons were inoculated which resulted in 7 well grown regenerated plants with a transformation rate of 1.35%. By PCR analysis, the SBEⅠantisense gene products were amplified in 5 regenerated plants which could provide evidence for the combination of SBEⅠantisense gene in cassava genome. The SBEⅠantisense gene can be successfully transformed to‘SC-8’cassava by the Agrobacterium-mediated transformation method. 5 transgenic‘SC-8’cassava plants were obtained.

References

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