Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (18): 127-131.doi: 10.11924/j.issn.1000-6850.2012-2275

Special Issue: 生物技术

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Isolation of CBF genes and their phylogenetic relationship with CBF genes in cereal family

  

  • Received:2012-06-25 Revised:2012-08-25 Online:2013-06-25 Published:2013-06-25

Abstract: A total of 11 clones were sequenced. Sequencing results showed that there were three copies of CBF genes in the 11 clones, among which similarities were 99%, 92% and 91% respectively. Moreover, the three cloned CBF genes were assigned with CBF genes from other different species. Assignment result showed that the cloned CBF genes of oil palm were clustered together. Moreover, there was higher similarity between the three cloned CBF genes of oil palm and HvCBF1, OsDREB1E, HvCBF11 as well as TaCBF11. Using homologous cloning, three CBF genes were cloned. By blast search, the cloned CBF genes have high similar with available CBF genes in NCBI website. The work will have a help for molecular selection of cold resistant variety in oil palm.