Abstract:

Using the areca catechu ‘Reyan No.1’ immature embryo as explants for embryo culture, using orthogonal design method to study the different medium, sucrose and active carbon concentration on Areca catechu embryo in vitro culture on the seedling growth effect. Through the analysis of variance and difference significance test, the best formula of combination evaluation, priority sequence of evaluation index considering was: the bud height→the longest root length→bud base diameter→root number. MS medium was the best in all medium, It can both satisfy the bud and root growth needs. Activated carbon significantly beneficial to take root and growth of root elongation, when used 4 g/L of activated carbon, the quantity of root and growth of root grew well, sucrose promoted root and bud growth, with the increase of adding amount of activated carbon, growth index showed low-high-low unimodal curve trend, 40 g/L sucrose concentration on bud growth best effect. This study concluded that the best formula of combination was: MS medium+ 4 g/L activated carbon+ 40 g/L sucrose..