Abstract:

The aim was to establish a rapid molecular assay for detection of Narcissus mosaic virus (NMV). A pair of specific primers was designed according to the published nucleotide sequence of NMV. The cDNA was synthesized from the total RNA prepared from narcissus sample and then PCR was carried out. The specificity, sensitivity, repeatability and practical effect of detection were validated. The method could specifically amplify an expected 483 bp fragment from NMV-infected narcissus sample, but not from healthy narcissus sample. The results showed that the method had strong specificity, high sensitivity and good repeatability. The method was successfully used to detect NMV on narcissus samples from port. It was concluded that RT-PCR provided reliable technical support for detection of NMV in Narcissus.