Abstract: A rapid and accurate method was established to simultaneously determine gallic acid and ellagic acid in Galla chinensis extracts. Full spectrum scan was adopted to examine the detection wavelength, factors that influence the determination results were studied to optimize the procedure, and the practicability of the determination method was analyzed. Samples were detected on the column of C18 (4.6 mm×250 mm, 5μm), eluted with acetonitrile and 10% glacial acetic acid as mobile phase in a gradient mode (0→10 min: 18% acetonitril, 10→15min: 18%→20% acetonitril). The procedure was at column temperature of 35℃, detection wavelength of 248 nm and flow rate of 1 mL/min. The working curves of gallic acid and ellagic acid were in the linear ranges of 0.44μg-1 mg, 0.08-8μg respectively, with R2 of 0.9995, with the average recovery of 96.74% (RSD=0.89% ) and 97.10% (RSD=1.53% ) respectively. The method can be employed to simultaneously determine the content of gallic acid and ellagic acid in Galla chinensis extracts within 15 minutes, which is proved a simple, rapid and accurate method in the process of high value utilization of Galla Chinensis with shortened measurement time and promoted efficiency.