Research Progress of Protoplast Culture in Woody Plants

doi:10.11924/j.issn.1000-6850.2012-2947

Abstract

Abstract: In order to provide theoretical basis for establishment of protoplast culture system and promote the work of rapid propagation and genetic improvement of woody plants, a detailed description about protoplast culture system in woody plants from three aspects such as separation, purification, culture method and medium composition was made in the article. Advantages and disadvantages in different types of separation and culture method of protoplast were analyzed. The main influence factors on protoplast culture in woody plants were summerized. Significance for distant hybridization, new variety creation and genetic improvement by the way of protoplast culture in woody plants was brought out. Research emphasis on protoplast culture in woody plants was also proposed.

PENG Shao-Feng chenyongzhong CHEN marry. Research Progress of Protoplast Culture in Woody Plants[J]. Chinese Agricultural Science Bulletin, 2013, 29(1): 1-6.

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