Abstract: Cinnamomum platyphyllum tissue culture and regeneration system was established based on two young stem segments for explants. Research different kinds, concentrations of growth regulators and illumination conditions on affect of cultivation each stage. The results showed that the appropriate media for the axillary bud induction and germinate from young stem segments was MS. The optimal medium for multiplication was MS+ BA 0.5 mg/L + NAA 0-0.05 mg/L, and the regenerating coefficient was above 3.5. The medium for the rooting was 1/2MS+ NAA 0.1 mg/L + IBA 0.2 mg/L, and the rate reached above 98%. Establish the rapid propagation of Cinnamomum platyphyllum providing the reliable technical basis for utilization and factory production.