Abstract: In order to purify crude kadsura polysaccharides(KPS) which were extracted from dried Kadsura marmorata fruits. The crude KPS were removed protein by trichloroacetic acid (TCA) method and Sevage method, respectively. On the base of single factor tests (decoloring temperature, decoloring time, solvent pH value and active carbon concentration), the conditions of active carbon decoloring method were optimized by the orthogonal test. The results showed that TCA method was the most appropriate removal protein method from the crude KPS and the ratio of removing protein reached to 72.6%. The optimal technological conditions of active carbon decoloring method was 60℃ of decoloring temperature, 80min of decoloring time, 1.0 of solvent pH value and 1.5% of active carbon concentration. In this condition, decoloring ratio reached to 86.83%. So the methods were simple, easy to apply, and suitable for the protein and pigment removal in crude KPS from K. marmorata fruits．