Abstract: In order to perfect the plant regeneration system of tissue culture, a detailed study was carried out to provide the experimental basis for genetic transformation by using embryonic leaflet. The peanut plantlets were regenerated from immature leaves, cotyledon, epicotyl and hypocotyls on MSB5 medium supplemented with 1 mg/L NAA and 6 mg/L BAP. After 10 days, callus was transferred onto MSB5 medium supplemented with 10 mg/L BAP or 3 mg/L BAP and 1 mg/L ABA. The results were follows: the frequency of middle sections of 6 days explant was 91.4% in contrast to epicotyls 26.7% and hypocotyls 12.5%. The frequency of 8823 was up to 91.4%. The root was induced and the plant was generated. Conclusion: Compared with the cotyledon, epicotyl and hypocotyls, the immature leaves was the most easiest to induce adventitious shoots. The optimum explants source was the middle sections of 6 days. The frequency of adventitious shoots depended on genotypes.